四、结论

在这项工作中，草莓视频在线播放研究了 16-E2-16 与 通过使用紫外-可见光、荧光和 CD 光谱技术结合计算方法来计算 Hb。 此外， 该相互作用系统也采用表面张力法。 在 UV-vis 研究的帮助下，草莓视频在线播放发现 16-E2-16 导致血红素基团暴露于水性介质。 荧光光谱证实内在猝灭 16-E2-16 的渗透抑制了血红素对 Trp 的影响 进入 Hb 的疏水腔并导致荧光强度增加。 效果还是比较明显的 只到cmc。 Trp 荧光比 Tyr 受到的影响更大 如同步光谱所示。 表面张力结果 确定 16-E2-16 的 cmc 值在存在的情况下增加 Hb 以及随着温度升高而 cac 保持不变 在所有温度下保持恒定。 表面张力测量也 表明胶束化过程是自发的、放热的和 熵驱动。 此外，得出结论，吸附在 界面优于胶束化。 分子建模方法表明 16-E2-16 在 Hb 中的结合 位于α1和β2链中。 它还提供了对草莓视频在线播放的验证 通过确认主要结合力的实验结果 是疏水的和静电的。 圆二色性表明 16-E2-16 与 Hb 结合导致 α-螺旋含量减少。 MD 模拟结果表明 Hb 和 16-E2-16 结合 Hb 稳定在 6000 ps 左右并确认构象 与 16-E2-16 相互作用后 Hb 发生了变化，如 草莓视频在线播放的 CD 结果显示。

致谢

Rajan Patel 博士非常感谢来自 科学与工程研究委员会（SR/S1/PC-19/2011 和 SB/EMEQ-097/2013) 和新德里大学拨款委员会， 印度（F.No. 39-841/2010 (SR)）。 Abbul Bashar Khan 博士表示感谢 印度新德里科学与工程研究委员会， 提供研究资助 (SB/FT/CS-031/2013)。 作者还 感谢 DST 为 FIST 拨款提供制裁令编号。 （SR/FIST/LS-541/2012）。

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酯功能化的双子表面活性剂与血红蛋白的结合——摘要、简介

酯功能化的双子表面活性剂与血红蛋白的结合——材料和方法

酯功能化的双子表面活性剂与血红蛋白的结合——结果和讨论

酯功能化的双子表面活性剂与血红蛋白的结合——结论、致谢！